| Add additional rows as needed for each
resource type |
Include species and sex when
applicable. |
Include name of manufacturer, company,
repository, individual, or research lab. Include PMID or DOI for
references; use “this paper” if new. |
Include catalog numbers, stock numbers,
database IDs or accession numbers, and/or RRIDs. RRIDs are highly
encouraged; search for RRIDs at https://scicrunch.org/resources. |
Include any additional information or notes
if necessary. |
| Antibody |
anti-Kmt2a primary antibody |
Bethyl |
374A |
|
| Antibody |
anti-Cas9 |
Millipore |
7A9 |
|
| Antibody |
β-Actin |
Ambion |
1406030 |
|
| Cell line |
human neural progenitor cells |
Donor from male control patient |
NSB2607 |
HiPSC-derived cell line. Collected as
fibroblasts, reprogrammed into NPCs. More information here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM2843554
|
| Chemical Compound or Drug |
Trizol |
ThermoFisher |
15596026 |
|
| Chemical Compound or Drug |
200 mg/ml THC stock |
NIDA |
N/A |
|
| Commercial Assay Or Kit |
RNAqueous-micro kit |
Ambion |
AM1931 |
|
| Commercial Assay Or Kit |
TaqMan assay |
ThermoFisher |
Rn01444748_m1 |
|
| Commercial Assay Or Kit |
nCounter array |
Nanostring |
N/A |
|
| Commercial Assay Or Kit |
DNAse treatment |
ThermoFisher |
EN0525 |
|
| Commercial Assay Or Kit |
Bioanalyzer |
Agilent |
G2939BA |
|
| Commercial Assay Or Kit |
uQuant plate reader |
Biotek Instruments |
UNK446 |
|
| Commercial Assay Or Kit |
NovaSeq 6000 S1 flowcell PE50 |
Illumina |
N/A |
|
| Commercial Assay Or Kit |
Qubit instrument |
ThermoFisher |
Q33239 |
|
| Deposited Data; Public Database |
Gene Expression Omnibus |
NCBI |
GSE87610, GSE102556
|
|
| Genetic reagent |
ON-TARGETplus SMART pool,
rat Kmt2a,
|
Horizon Discovery |
L-100995–02-0005 |
|
| Genetic reagent |
ON-TARGET nontargeting pool |
Horizon Discovery |
D-001810–10-05 |
|
| Genetic reagent |
lentiCRISPR v2 |
Addgene |
plasmid # 52961 |
|
| Genetic reagent |
Guide RNAs |
Benchling |
N/A |
|
| Genetic reagent |
AAV8-hSyn-Cre-GFP |
https://www.med.unc.edu/genetherapy/vectorcore/in-stock-aav-vectors/reporter-vectors/
|
N/A |
|
| Transfection Reagent |
JetSI transfection reagent |
Polyplus Transfection |
N/A |
|
| Organism/Strain |
Long-Evans female dams |
Charles River Laboratories |
N/A |
|
| Organism/Strain |
Mice (Kmt2a2lox/2lox) |
Bred in-house |
|
|
| Software; Algorithm |
Gen5 |
Biotek Instruments |
RRID:SCR_017317 |
|
| Software; Algorithm |
Subread |
PMID:23558742 |
RRID:SCR_009803 |
|
| Software; Algorithm |
DESeq2 |
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
RRID:SCR_015687 |
|
| Software; Algorithm |
Voom-limma |
http://bioinf.wehi.edu.au/limma/
|
RRID:SCR_010943 |
|
| Software; Algorithm |
Bowtie |
PMID:19261174 |
RRID:SCR_005476 |
|
| Software; Algorithm |
SAMtools |
PMID:21903627 |
RRID:SCR_002105 |
|
| Software; Algorithm |
MACS |
PMID:18798982 |
RRID:SCR_013291 |
|
| Software; Algorithm |
FastQC |
http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
|
RRID:SCR_014583 |
|
| Software; Algorithm |
diffReps |
https://doi.org/10.1371/journal.pone.0065598
|
RRID:SCR_010873 |
|
| Software; Algorithm |
ChIPseeker |
http://bioconductor.org/packages/devel/bioc/vignettes/ChIPseeker/inst/doc/ChIPseeker.html#:~:text=ChIPseeker%20is%20an%20R%20package,peaks%20binding%20to%20TSS%20regions.&text=Currently%2C%20ChIPseeker%20contains%2017%2C000%20bed%20file%20information%20from%20GEO%20database. |
N/A |
|
| Software; Algorithm |
HISAT2 |
PMID:25751142 |
RRID:SCR_015530 |
|
| Software; Algorithm |
STRING |
PMID:21045058 |
RRID:SCR_005223 |
|
