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. Author manuscript; available in PMC: 2022 Mar 27.
Published in final edited form as: J Alzheimers Dis. 2017;59(2):743–751. doi: 10.3233/JAD-170298

Fig. 4.

Fig. 4.

Tau monomers trigger calpain-mediated generation of a 17 kDa tau fragment. A) Representative western blotting of brain extracts from mice injected with control ACSF solution or tau peptides (ICV for 3 h) using calpain 1 and spectrin antibodies for detection. Densitometric quantification shows that the levels of the two enzymes are significantly increased by mTau but not oTau. B) Representative western blotting of brain extracts from mice injected with control ACSF solution or tau peptides (ICV for 3 h) using Tau 1 antibody for detection. Only after treatment with monomeric preparations was present a band at approximately 17 kDa molecular weight. In all cases an antibody raised against GAPDH served as a loading control. The data are mean ± SEM, *p < 0.05; **p < 0.01 versus control by one-way ANOVA followed by Bonferroni post hoc test, n = 6.