TABLE 1.

human CD47 expression, other transgenes (Tg), immunosuppression, urine protein and survival of recipients after GalT-KO+Tg K+VT XTx

			hCD47 in graft kidney (High, Low or No)		Other Tgs		Immunosuppression			U-proteinuria				Survival
Group	Distribution of hCD47 Expression in the donor kidneys	Baboon ID	Glomeruli	Tubules	Human CRP	Other Tg	Anti-CD40L mab or anti-CD40 mab	Anti-IL6R Ab	CTLA4-lg Rescue therapy After 2+ proteinuria	1+ or less throughout	2+	3+	Reverse to 1+	POD	Avg	Cause of euthanasia and sCre at the end point
A	HIGH/LOW Glomerular &NO Tubular expression of hCD47	12P86	High	NO	CD55	NO	Anti-CD40L	NO	NO	POD 1-128	NO			128	128	Graft growth, Cr 1 mg/dl
		14P9	Low	NO						NO	POD 47-68	NO		68	68	Pleural effusion, Cr 2.8 mg/dl
B	HIGH Glomerular & HIGH Tubular expression of hCD47	1615	High	High	CD55	NO	Anti-CD40	NO	NO	POD 1-48	POD 49-50	NO		50	51.5	Tracheal edema Cr 1.6 mg/dl
		2415	High	High						POD 1-53	NO			53		Tracheal edema Cr 2.1 mg/dl
		14P18	High	High	CD46, CD55	CMAHKO, B4KO, EPCR, TBM, HO-1	Anti-CD40	YES	NO	POD 1-154	NO			154	170.5	Graft growth Cr 2.8 mg/dl
		14P66	High	High						POD 1-185	POD 186-187	NO		187		MMF crystallization in SVC, Cr 0.9 mg/dl
C	LOW/NO Glomerular & HIGH Tubular expression of hCD47 (Rescue CTLA4-lg)	13P60	NO	High	CD46	EPCR	Anti-CD40	NO	POD 16	NO	POD 15-42	NO		42*	56*	Kidney graft excised from this animal on POD 42 for studies unrelated to this project Cre 2.0mg/dl
		13P79**	NO	High		TFPI			POD 22	NO	POD 21-36	NO	POD 37-70	70		**IBBMTx delayed KTx, Pneumonia, Cr 0.8 mg/dl
		14P56**	NO	High		TFPI			POD 24	NO	POD 17-34	NO	POD 35-41	41		**IBBMTx delayed KTx. Pneumonia, Cr 0.9 mg/dl
		14P65	Low	High	CD46	CMAHKO, TBM, EPCR	Anti-CD40	YES	POD 17	NO	POD 15-80	NO	NO	80	80	Baboon CMV.Cr 0.8 mg/dl

^*Excluded 13P60 from the average because the kidney graft was removed for studies unrelated to this project.

^**Baboons that received IBBMTx one month prior to kidney Tx. No VT grafts were co-transplanted.