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. Author manuscript; available in PMC: 2022 Mar 27.
Published in final edited form as: Cell Rep. 2022 Mar 8;38(10):110467. doi: 10.1016/j.celrep.2022.110467

Figure 4. Characterization of coordinated gene expression and chromatin accessibility programs in human pituitary cell types.

Figure 4.

(A) Top, Heatmaps of gene expression levels for the top 200 genes associated with the human PSC LV (LVscrna, gray vertical line, top 30 genes highlighted in C). Bottom, Heatmap of chromatin accessibility levels for the top 200 genes associated with the human LVscrna applied to the human snATAC-seq datasets. Cell-type-associated boxplots are shown for each heatmap.

(B) Heatmap for the top 200 genes associated with the human LVscrna applied to the murine snRNA-seq dataset (top), and for the top 200 genes associated with the human LVscrna applied to the murine snATAC-seq datasets (bottom) (Ruf-Zamojski et al., 2021). Cell-type-associated boxplots are shown for each heatmap.

(C) Heatmap of gene expression levels for the top 30 genes associated with human PSC heatmap LVscrna (A). (A–C) Each pituitary sample is indicated at the top. In the scale bars, red signifies the highest level of RNA expression or chromatin accessibility. Pd, pediatric; Ad, adult; Ag, aged pituitary. Cell type and donor color-coding are provided on the bottom key. (A, B) The blue dotted horizontal line corresponds to the top 30 genes as shown in (C).

(D) Chromatin accessibility tracks for LHX3 (identified in C, box) for all cell types in the pediatric female sample. The gene structure is provided underneath the tracks. Additional tracks are presented in Figure S5. Refer to Table 1 for donor-related information. Additional LV analyses are presented in Figure S4. The top 200 genes for all LVs illustrated in Figure 4 are provided as spreadsheets in the Supplemental information.