KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological samples | ||
| Female pediatric pituitary (frozen from deceased) | NIH Neurobiobank | #1275, NDAR_INVCK755EF3 |
| Female adult pituitary (frozen from deceased) | NIH Neurobiobank | #5621, NDAR_INVXK717LJA |
| Female aged pituitary (frozen from deceased) | NIH Neurobiobank | #187443 |
| Male pediatric pituitary (frozen from deceased) | NIH Neurobiobank | #1674, NDAR_INVMV348UPL |
| Male adult pituitary (frozen from deceased) | NIH Neurobiobank | #5818, NDAR_INVKF350UGX |
| Male aged pituitary (frozen from deceased) | NIH Neurobiobank | #187438 |
| Wildtype murine postnatal pituitaries | Charles River Laboratories | CD-1 strain; MGI: 5649524 |
| Chemicals, peptides, and recombinant proteins | ||
| RNAse inhibitor | NEB | # MO314L |
| OptiPrep | StemCell Tech | 07820 |
| Sucrose | Sigma | S0389 |
| EDTA | Corning | 46-034-Cl |
| Tris-HCl, pH 7.4 | Sigma | T2663 |
| CaCl2 | Sigma | 21115 |
| Mg(Ac)2 | Boston Bioproducts | MT-190 |
| IGEPAL CA-630 | Sigma | I3021 |
| 10% Buffered Neutral Formalin | Sigma | HT501128 |
| Mayer’s hematoxylin | Vector | H-3404 |
| Vectamount | Vector | H-5000 |
| Tween-20 Surfact Amps Detergent | Thermo Scientific | 85113 |
| Buffer EB | Qiagen | 19086 |
| SPRI Select | Beckman Coulter | B23318 |
| Critical commercial assays | ||
| Chromium Single Cell 3′ Reagents | 10x Genomics | V3 |
| Chromium Single Cell ATAC Reagents | 10x Genomics | V1 |
| Chromium Single Cell Multiome ATAC and Gene Expression Reagent | 10x Genomics | V1 |
| RNAscope 2.5 HD Duplex assay | Advanced Cell Diagnostics | N/A |
| Deposited data | ||
| Raw and analyzed data | This paper | GEO: GSE178454 |
| Raw and analyzed data | Ruf-Zamojski et al. (2021) | GEO: GSE151962 |
| Experimental models: Organisms/strains | ||
| Wildtype murine postnatal pituitaries | Charles River Laboratories | CD-1 strain; MGI: 5649524 |
| Oligonucleotides | ||
| Mm-Jun probe | Advanced Cell Diagnostics | # 453561 |
| Mm-Sox2-C2 probe | Advanced Cell Diagnostics | # 401041-C2 |
| Software and algorithms | ||
| Cell Ranger | 10x Genomics | v5.0.0 |
| Seurat | Butler et al. (2018) and Stuart et al. (2019) | v.3.9.9.9024; v4.0.1; v3.1.5 |
| Cell Ranger-ATAC pipeline | 10x Genomics | v1.2.0 |
| Seurat/Signac | Stuart et al. (2020) | v3.1.5/0.2.4 |
| Cell Ranger ARC | 10x Genomics | v1.0.0 |
| Monocle3 | Cao et al. (2019) | v0.2.3.0 |
| PLIER | Mao etal. (2019) | N/A |
| Original code for linear model | This paper | https://github.com/zidong93/snpituitary_human_manuscript |
| Other | ||
| Dounce glass homogenizer | VWR | # 71000-514 |
| 40 mm cell strainer | Falcon | 352340 |
| Centrifuge SW41 rotor | Beckman Coulter | #331362 |
| Fluorometer | Invitrogen | Qubit3 |
| Bioanalyzer | Agilent | 2100 |
| Sequencer | Illumina | MiSeq |
| Sequencer | Illumina | NovaSeq 6000 |
| Cell counter | Nexcelome | K2 |
| Cell Counter | Invitrogen | Countess |