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. 2022 Mar 8;9:813215. doi: 10.3389/fcvm.2022.813215

Figure 2.

Figure 2

Mice with combined hyperlipidemia and hyperglycemia show larger plaques than hyperlipidemic mice. (A–D) Representative histological images showing cross-sections of aortic sinus stained with Hematoxylin/ Eosin [H & E, (A)] and bar graphs summarizing data (B). Representative histological images showing aortic sinus sections stained with Oil-red O (C) and bar graphs summarizing data (D). (E–H) Representative histological images showing truncus brachiocephalic arteries (BCA) sections stained with Oil-red O (E) and bar graphs summarizing data (F). Stainings were imaged at 4 × magnification (scale bar 100 μm) (A,C,E). HFD control 12 and 20 weeks (N = 7), HCi HFD 12 and 20 weeks (N = 5), and HGHCi HFD 12 and 20 weeks (N = 6). Determination of the external elastic lamina (EEL) (G), internal elastic lamina (IEL) (H), media area (I) and the lumen area (J) from Oil-red O stained cross-sections of the BCA (see Supplementary Figure II). Data presented as mean ± SEM and two-way ANOVA were performed with Sidak's multiple comparison post-hoc test (*p < 0.05). Control HFD: Wild type mice without rAAV8-PCSK9D377Y injection on high fat diet (HFD); HCi HFD: rAAV8-PCSK9D377Y injection plus HFD (hyperlipidemic); HGHCi HFD: rAAV8-PCSK9D377Y and streptozotocin injection and HFD (hyperlipidemic and hyperglycemic).