FIGURE 2.

Characteristics of cultured basal-like cells. scRNA-seq was performed in basal-like cells derived from three different IPF patients, and the expression levels of the basal cell markers KRT5 and KRT17, proportional to the size and color intensity of the dots, are shown on a t-SNE plot (A). t-SNE shows the best matching cell type for each cell after annotation of cultured cells using as reference an scRNA-seq atlas of cell types in the human lung (B). KRT5 (n = 11) and KRT17 (n = 5) RNA expression was confirmed by TaqMan PCR (C). Representative images showing staining for KRT5 (red), left, and KRT17 (green), middle, and their merged image, right in cultured basal-like cells of four different IPF patients (D).