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. 2022 Mar 8;10:844119. doi: 10.3389/fbioe.2022.844119

FIGURE 3.

FIGURE 3

Anti-fibrotic effects of basal-like cell-CM on fibroblasts in vitro. Fibroblasts were treated with the control medium, basal-like cell-conditioned medium (CM), or fibroblasts-CM (F-CM). α-SMA RNA expression was measured by TaqMan RT-PCR (n = 3) (A), and changes in α-SMA protein in fibroblasts (n = 3) treated with basal-like cell-CM of six different patients (1–6) were determined by immunoblotting (B). Col1A1 RNA expression was determined after 24 h (n = 7) (C) and Col1A1 deposition after 48 h (n = 5) (D). MMP-1 or TIMP-1 RNA expression (E,G) or protein secretion (F,H) was measured after 24 h. Data are expressed as fold change or as a percentage of fibroblasts treated with control medium (RNA) or in ng/ml (protein). HGF in supernatants of fibroblasts (n = 12) treated with basal-like cell-CM, control medium, or in basal-like cell-CM before the addition to fibroblasts (basal-like cell-CM only) (I).*p < 0.05.