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. 2022 Mar 8;10:844119. doi: 10.3389/fbioe.2022.844119

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Copyright © 2022 Khan, Fytianos, Blumer, Roux, Gazdhar, Savic, Knudsen, Jonigk, Kuehnel, Mykoniati, Tamm, Geiser and Hostettler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of different recombinant cytokines and PGE2 on fibroblasts in vitro. Fibroblasts were treated with eight different recombinant human cytokines (all at 10 ng/ml) or with PGE2 (1–1,000 nM) for 24 h. HGF secretion was measured in cell supernatants, and data were expressed as a percentage of fibroblasts treated with the control medium (A,B). Col1A1 (C,D), MMP-1 (E,F), and TIMP-1 (G,H) RNA expression was determined by TaqMan RT-PCR, and values were expressed as fold change of fibroblasts treated with the control medium. *p < 0.05.