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. 2022 Mar 8;10:844119. doi: 10.3389/fbioe.2022.844119

FIGURE 6.

FIGURE 6

PGE2 mediates basal-like cell-CM-induced HGF in IPF fibroblasts. PGE2 in supernatants of fibroblasts (n = 6) treated with basal-like cell-CM, control medium, or basal-like cell-CM before the addition to fibroblasts (A). PGE2 secretion from basal-like cells with and without COX inhibitor indomethacin (1 and 10 μM) (B). Data are expressed as pg/ml. IPF fibroblasts (n = 4) were incubated with the control medium or basal-like cell-CM collected from basal-like cells treated with or without indomethacin (1 and 10 μM). After 24 h, HGF secretion was measured in the cell supernatants, and data were expressed as a percentage of fibroblast HGF secretion treated with basal-like cell-CM alone (C). IPF fibroblasts (n = 4) were treated with the control medium or basal-like cell-CM with or without PGE2 receptor antagonists PF04418948 [(D), 1 and 10 μM] or AH6809 [(E), 20 μM]. HGF secretion was measured, and values are expressed as a percentage of fibroblast HGF secretion treated with basal-like cell-CM (D, E). *p < 0.05.