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. 2022 Mar 8;12:764711. doi: 10.3389/fcimb.2022.764711

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Copyright © 2022 Rashid, Correia-Mesquita, Godoy, Omran and Whiteway

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Invasiveness of SAGA mutants in C. albicans. Overnight cultures from fresh single colonies were grown in liquid YPD at 30°C and 220 rpm and diluted to an OD600 of 1. 3μL samples were spotted on YPD agar plates and Spider media plates and incubated at 30°C and 37°C for 120h. The resulting colonies were then washed gently under running water for 15 seconds to remove the non-adherent surface cells, and the invasiveness of the samples was observed. (A, B) The spt7Δ/Δ and spt8Δ/Δ knock-out mutants were found to be invasive. However, ngg1Δ/Δ and ubp8Δ/Δ were non-invasive in both conditions compared to its control. BW-Before wash; AW-After Wash.