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. 2022 Mar 8;10:794037. doi: 10.3389/fbioe.2022.794037

FIGURE 5.

FIGURE 5

HA inhibits miR-139-5p expression by activating CD44. (A–C). Western blot assays and qPCR assays (D,E) were performed to test CD44 and miR-139-5p expression after HA treatment in MSCs, ECFCs and MSCs-ECFCs, respectively. (F). Relative miR-139-5p expression in ECFCs transfected with CD44 overexpression plasmid. (G). qPCR assays were performed to test the CD44 and miR-139-5p mRNA expression in ECFCs transfected with CD44 siRNA, with or without administration of HA, respectively. (H). Flow cytometry of CD31 sorted from MSC-ECFC co-cultured system, with (red) or without (grey) administration of HA, and quantification of flow cytometry. (I). Relative miR-139-5p expression in the sorted CD31+ cells and CD31−cells, respectively. (n = 3 independent experiments. Error bars: mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001).