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. 2022 Mar 8;13:853317. doi: 10.3389/fphys.2022.853317

FIGURE 6.

FIGURE 6

Schematic of the experimental proof-of-concept protocols for inhalation assays. Exposure is limited first to the nasal compartment, either via direct instillation of the virus or nebulized Poly I:C viral simulant. Following exposure, all three apical compartments are connected in series to airflow supplied via a peristaltic pump. Note that airflow to the acinar compartment is reduced to a physiologically relevant range in the deep lungs (see Table 1). Each compartment is analyzed separately for viral quantity, deposition sites, and cellular cytokine secretion to the basal media.