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. 2022 Mar 8;13:853317. doi: 10.3389/fphys.2022.853317

FIGURE 9.

FIGURE 9

Non-replicating viral transmission simulation using poly I:C and subsequent IL-6 secretions across the three compartments. (A) Schematic of the nebulization setup used to supply aerosolized poly I:C to the multi-compartment model. Following exposure, cells are freeze-burst and their media collected for (B) differentiated quantification of inflammatory cytokines in each compartment. Interleukin 6 (IL-6) was measured via enzyme-linked immunosorbent assay (ELISA). In all models, an elevation of IL-6 levels can be seen upon exposure to poly I:C compared to PBS exposure as a control. Bars represent the average and error bars of the standard deviation (SD) (N = 3 for PBS, N = 5 for poly I:C).