Increases in local and systemic type 2 immune responses after NAC and EEC. Log10 fold increases in IL-5 (A) and IL-13 (B) gene expression from nasal brush samples collected at baseline and 8 hours after NAC (blue) or baseline and 8 hours after the second day of EEC (red). Levels of IL-5 (C) and IL-13 (D) in nasal fluid collected prechallenge and up to 8 hours after allergen challenge. Serum IL-5 and IL-13 concentrations prechallenge and 6 hours after NAC (E and F) or prechallenge day 1, prechallenge day 2, and 6 hours after EEC day 2 (G and H). NS, Not significant.*P < .05, **P < .01, ***P < .001, ****P < .0001.