Fig. 6.
Genotoxic stress induces C16-HSL release into the supernatant through a prophage gene
(A, B) HPLC-MS analysis of C16-HSL extracted from (A) total supernatants or (B) MVs. n=3; mean±s.d. *: P<0.05, ns: P≥0.05 (Welch’s t-test). (C) Bioassay for QS promoter activity. The AHL biosensor Paracoccus denitrificans Pd1222ΔpdnIΔpxm/pPLlas was cultivated using the total supernatant of P. denitrificans Pd1222 cultured with or without MMC. Results are expressed as a fold change in fluorescence intensity relative to that in MMC-untreated cells. n=3; mean±s.d. *: P<0.05, ns: P≥0.05 (Welch’s t-test).