FIGURE 4.

Myeloid and hepatocyte MD2 is required for lipid accumulation in mice fed a high‐fat diet (HFD). (A) The schematic diagram showing the grouping and steps of animal experiments. WT and MD2KO mice were irradiated and received bone marrow cells from either WT mice or MD2KO mice (WT→WT = marrow cells from WT mice transplanted in irradiated WT mice; MD2KO→WT = marrow cells from MD2KO mice transplanted in irradiated WT mice; WT→MD2KO = marrow cells from WT mice transplanted in irradiated MD2KO mice; MD2KO→MD2KO = marrow cells from MD2KO mice transplanted in irradiated MD2KO mice). All transplanted mice were fed an HFD for 16 weeks. (B) Body weight of mice recorded weekly. (C) Liver weight relative to BW (LW/BW) ratios at the time of harvest. (D–F) Serum levels of total cholesterol (TCH) (D), low‐density lipoprotein‐cholesterol (LDL‐C) (E) and high‐density lipoprotein‐cholesterol (HDL‐C) (F) in mice after marrow reconstitution and HFD feeding for 16 weeks. (G and H) Hematoxylin and eosin (H&E) staining of liver tissues (G) and non‐alcoholic fatty liver disease (NAFLD) activity score analysis (H). (I and J) Lipid accumulation in liver tissues as detected by oil red O staining (I) (scale bar = 100 μm). Quantification of lipid area is shown in panel (J). In panel (C–J), n = 6 per group; data are shown in mean ± SEM; ns, not significant; *p < .05, **p < .01 and ***p < .001