FIGURE 4.

Deficiency of GPR65 in CD4⁺ T cells restrains trinitrobenzene sulfonic acid (TNBS)‐induced acute colitis in mice. TNBS‐induced acute colitis was established in Gpr65^ΔCD4 and Gpr65^flx/flx mice (n = 10/group). (A and B) Changes in body weight and disease activity index (DAI) in these mice were recorded daily after TNBS enema. (C and D) Gpr65^ΔCD4 and Gpr65^flx/flx mice were sacrificed on day 7 after administration of TNBS rectally, and colon morphology and length were shown. (E) Histological scores of colonic tissues as indicated. (F) Representative colon sections were stained with H&E. Scale bars 200 μm. (G to J) Intracellular expression of IFN‐γ, IL‐17A and TNF‐α was analysed by flow cytometry in LP‐CD4⁺ T cells after stimulated with PMA and ionomycin for 5 h, the percentages and numbers of IL‐17A⁺, IFN‐γ ⁺, and TNF‐α⁺CD4⁺ T cells were shown in the bar chart. Statistical analyses were performed with unpaired Student's t tests. Data were presented as mean ± SEM. Data were representative of three independent experiments. Statistical analysis was performed using Tukey's multiple comparison test (A, B, and D), and multiple t test (E, F, I and J). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001