Fig. 1.

(A) Flow cytometry analysis of CD301b⁺ macrophages in the tissues surrounding BCP bioceramics at 14 days after implantation. (B) Semiquantification of gating strategy cells in (A). ***P < 0.001. (C) IHC staining of regenerative macrophages subsets CD301b and α-SMA after BCP bioceramics implantation at 7 and 14 days (the red arrow showed the positive cells; M, material). Scale bar = 200 μm. (D) IHC staining of α-SMA in BCP implant areas after CD301b⁺ macrophages depletion at 14 days (the red arrow showed the positive cells; M, material). Scale bar = 100 μm. (E) Semiquantification of positively stained cells in (D). **P < 0.01. (F) IF staining of VEGF (red), CD301b (green) and nuclei (blue) at 14 days after BCP bioceramics implantation in vivo. (Scale bar = 100 μm). (G) IHC staining of VEGF in BCP implant areas after CD301b⁺ macrophages depletion at 14 days (the red arrow showed the positive cells; M, material). Scale bar = 100 μm. (H) Semiquantification of positively stained cells in (G). **P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)