Table 1.
An mmf1Δ mutant has decreased heme levels.
| Heme levelsa | |||||
|---|---|---|---|---|---|
| Medium | |||||
| Strain | Genotype | SD | SD + Ile | SD + ALA | YPD |
| YJF153 | YJF153 | 254 ± 29 | 238 ± 10 | 251 ± 26 | 258 ± 26 |
| Dmy41 | YJF153 mmf1Δ | 90 ± 10 | 241 ± 13 | 157 ± 13 | 80 ± 7 |
| Dmy74 | YJF153 hem1Δ | ND | ND | 124 ± 26 | ND |
| Dmy61 | S288c | 140 ± 25 | 186 ± 39 | 176 ± 12 | 217 ± 8 |
| Dmy67 | S288c mmf1Δ | 84 ± 8 | 176 ± 19 | 125 ± 4 | 65 ± 9 |
Mutants lacking MMF or HEM1 in each of two strain backgrounds (YJF153 and S288c) were grown in the indicated medium. When added, L-isoleucine and aminolevulinic acid were at 1 mM and 240 μM, respectively. Data are from a representative experiment with three biological replicates and reported as average plus or minus 1 standard deviation. Abbreviations: SD, synthetic dextrose; YPD, yeast peptone dextrose; Ile, L-isoleucine; ALA, aminolevulinic acid.; ND, not determined.
a
Heme levels were measured as described in Materials and Methods and are reported in pmol/OD650.