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. 2022 Jan 25;16:149–161. doi: 10.1016/j.bioactmat.2022.01.031

Fig. 2.

Fig. 2

Adipogenic and chondrogenic induction of cells on scaffolds. (A) Stem cells seeded on NS, CNS1, CNS2, or CNS3 for adipogenic and chondrogenic induction; (B) Proliferation assays of bone marrow stem cells (BMSCs) on the scaffolds on days 1, 4, and 7; (C) immunofluorescent staining of col2a1 in BMSCs seeded on the scaffolds after 14 d of induction; (F) immunofluorescent staining of PPARγ and oil red O of 3T3-L1 cells seeded on the scaffolds after 14 d of induction. Significant difference compared with the NS (*), CNS1 (+), and CNS2 (#) groups, P < 0.05; significant difference compared with the NS (**), CNS1 (++), and CNS2 (##) groups, P < 0.01; NS, nanofiber scaffold; CNS, crimped nanofiber scaffold.