Fig. 4.

Histological, immunohistochemical, and chondrogenic-related gene expression evaluation of tendon-to-bone regeneration. (A) Safranin O/Fast Green staining and (B) immunohistochemical staining of collagen II; (C–D) quantitative analysis of in vivo tendon-to-bone interface regeneration. Significant difference compared with the defect (*), autograft (⁺), and NS (^#) groups, P < 0.05; Significant difference compared with the NS (**), CNS1 (⁺⁺), and CNS2 (^##) groups, P < 0.01; 1 M, 1 month; 3 M, 3 months; B, bone; CNS: crimped nanofiber scaffold; I, interface; NS, nanofiber scaffold; T, tendon. Red dotted shapes indicate the regenerated tendon-to-bone interface. Black arrows indicate collagen II expression.