Evaluation of fatty infiltration. (A) Representative oil red O images of rotator cuff muscle; (B) schematic illustration of CNS3 used for MRCT repair; (C–E) adipogenic-related gene expression in the supraspinatus tendon. Significant difference compared with the defect (*), autograft (+), and NS (#) groups, P < 0.05; Significant difference compared with the NS (**), CNS1 (++), and CNS2 (##) groups, P < 0.01; MRCT, massive rotator cuff tear; CNS, crimped nanofiber scaffold; NS, nanofiber scaffold.