Fig. 9.

H-HPMA bottlebrush hydrogel ameliorates mesothelial-to-mesenchymal transition in rat abdominal adhesion model (A) Images of immunofluorescence staining on day 10 after adhesiolysis. Sections of tissue were stained with DAPI (blue), occludin-Alexa Fluor® 594 (red) and ZO-1-Alexa Fluor®488 (green). Magnification: 200 × ; Scale bars represent 100 μm in the lower magnification images and 50 μm in the higher magnification images. (B-C) Relative mRNA expression of the fibronectin and collagen-Ⅰ in the adhesive site of different groups on day 5, day 10 after first surgery and day 5 after adhesiolysis. All data are presented as mean ± SD (n = 3 per group); the ns means no significant difference; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.