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. 2022 Mar 3;60:101468. doi: 10.1016/j.molmet.2022.101468

Supplemental Figure 4.

Supplemental Figure 4

Expression of Wt-SOD, Mt-SOD, Wt-TDP43, or Mt-TDP43 in neurons does not significantly alter mitochondrial respiration and extracellular acidification rates. Panel A. ECAR profile of LacZ, Wt-SOD, and Mt-SOD expressing neurons monitored under basal condition and upon the sequential injections of oligomycin A, CCCP, Antimycin A/Piericidin A, and 2-DG (injections are indicated by arrows). Panel B. Basal ECARs of LacZ, Wt-SOD, and Mt-SOD expressing neurons. Panel C. ECARs of LacZ, Wt-SOD, and Mt-SOD expressing neurons upon the injection of oligomycin A. Panel D. ECAR profile of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons monitored under basal condition and upon the sequential injections of oligomycin A, CCCP, Antimycin A/Piericidin A, and 2-DG (injections are indicated by arrows). Panel E. Basal ECAR of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons. Panel F. ECARs of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons upon the injection of oligomycin A. Panel G. OCR profile of LacZ, Wt-SOD, and Mt-SOD expressing neurons monitored under basal condition and upon the sequential injections of oligomycin A, CCCP, Antimycin A/Piericidin A, and 2-DG (injections are indicated by arrows; injection of 2-DG not shown). Panel H. Basal OCRs of LacZ, Wt-SOD, and Mt-SOD expressing neurons. Panel I. OCR profile of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons monitored under basal condition and upon the sequential injections of oligomycin A, CCCP, and Antimycin A/Piericidin A, and 2-DG (injections are indicated by arrows; injection of 2-DG not shown). Panel J. Basal OCRs of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons. Panel K. Maximal OCRs of LacZ, Wt-SOD, and Mt-SOD expressing neurons upon the injection of CCCP. Panel L. ATP-production coupled OCRs of LacZ, Wt-SOD, and Mt-SOD expressing neurons. Panel M. Maximal OCRs of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons upon the injection of CCCP. Panel N. ATP-production coupled OCRs of LacZ, Wt-TDP43, and Mt-TDP43 expressing neurons. Panels A, D, G, and I are representatives of 4 biologically independent experiments with an n = 6 technical replicates. For B, C, E, F, H, and J-N, n = 4 biologically independent experiments. Data are mean ± s.e.m. (A-N). One-way ANOVA with Dunnett's T3 multiple comparisons tests (α = 0.05) were used for determining statistical significance.