Figure 2.

Metabolic interrogation of glycolysis and related pathways in neurons expressing Wt and fALS Mt genes. Pooled data from 3 to 5 experiments comparing neurons expressing LacZ with Wt SOD1/TDP43 with Mt SOD1/TDP43 is presented. Horizontal lines denote means ± SD. ANOVA showed group differences, and values above the scattergram indicate that in post hoc tests the specific group is statistically significantly different from the LacZ group (p value provided) or not different (NS – not significant). Values below the scattergram indicate that the Wt or Mt proteins group are statistically significantly different or not. Panel labels: A, B, Net glucose uptake during the 4 h incubation; C, D, total lactate release to medium; E, F, ¹³C enrichment of medium lactate; G, H, Flux of glucose through the pentose phosphate pathway (PPP); I, J, ¹³C enrichment of intracellular ribose-5-phosphate (P); K, L, ¹³C enrichment of intracellular acetylglucosamine. For the CMR_glc summary panels (M and O), data are means and 1 SD, with all rates in units of glucose or its equivalent (i.e., lactate/2). For the pathway rates expressed as percent of the LacZ rate or of CMR_glc (Panels N and P), values are mean quotient and vertical bars are CVs for the 100% rates or SDs that account for error propagation for all others. Statistically significant changes are indicated in the panels that illustrate the primary data.