Key resources table.
| Reagent or Resource | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-MAP2 | Abcam | Cat.No. ab5392 RRID AB_2138153 |
| Mouse monoclonal anti-Piccolo | Synaptic Systems | Cat.No. 142111, clone 68G2, RRID AB_2619829 |
| Rabbit polyclonal anti-Glia1 | Synaptic Systems | Cat.No. 182003 RRID AB_2113441 |
| Guinea pig polyclonal anti-Glia2 | Synaptic Systems | Cat.No. 182105 RRID AB_2619875 |
| Mouse monoclonal anti-GluN1 | Synaptic Systems | Cat.No. 114011 RRID AB_887750 |
| Mouse monoclonal anti-PSD95 | NeuroMab (UCDavis) | Clone K28/43, RRID:AB_2292909 |
| Rabbit polyclonal anti-S100 beta | LSBio | Cat.No. LS-C268728 RRID:AB_11143806 |
| Mouse monoclonal anti-GFAP | Synaptic Systems | Cat.No.173211 RRID:AB_2232308 |
| Mouse monoclonal anti-neurofilament H, non-phosphorylated clone SMI 32 | BioLegend | Cat.No. 801701 RRID AB_2314912 |
| Mouse monoclonal anti-glial fibrillary protein | Synaptic Systems | Cat.No. 173211, clone 186C6, RRID AB_2721090 |
| Polyclonal rabbit anti-glucose-6-phosphate dehydrogenase | Abcam | Cat. No. ab993 RRID AB_296714 |
| Rabbit monoclonal anti-AMPK | Cell Signaling | Cat.No.2532S RRID AB_10624867 |
| Rabbit monoclonal anti-phosphoAMPK | Cell Signaling | Cat.No.2535S RRID AB_331250 |
| Rabbit monoclonal anti-ACC | Cell Signaling | Cat.No.3676SAB RRID AB_2219397 |
| Rabbit monoclonal anti-phosphoACC | Cell Signaling | Cat.No.3661S RRID AB_330337 |
| Rabbit monoclonal anti-4EBP1 | Cell Signaling | Cat.No.9452 RRID AB_331692 |
| Rabbit monoclonal anti-phospho4EBP1 | Cell Signaling | Cat.No.2855S RRID AB_560835 |
| Mouse monoclonal anti-HA (clone 16B12) | BioLegend | Cat.No. 901514 RRID:AB_2563417 |
| Rabbit polyclonal anti-FLAG | Sigma Aldrich | Cat.No. 60-031 RRID:AB_10718939 |
| Polyclonal goat anti-guinea Pig IgG (H + L), Alexa Fluor 488 | Life Technology | Cat.No. A-11073 RRID:AB_2534117 |
| Polyclonal goat anti-mouse IgG (H + L), Alexa Fluor 594 | Life Technology | Cat.No. A11032 RRID:AB_2534091 |
| Polyclonal goat anti-rabbit IgG (H + L), Alexa Fluor 488 | Life Technology | Cat.No.A11034 RRID:AB_2576217 |
| Polyclonal goat anti-chicken IgG (H + L), Alexa Fluor 488 | Life Technology | Cat.No.A11039 RRID AB_2534069 |
| Bacterial and virus strains | ||
| HSV-WT-superoxide dismutase (SOD1) | Lim et al., 2012 | |
| HSV-G86R SOD1 | Lim et al., 2012 | |
| HSV-WT-TAR DNA binding protein 43 (TDP43) | Jablonski et al., 2015 | |
| HSV-M337V TDP43 | Jablonski et al., 2015 | |
| HSV-miRNA to G6PDH | This study | |
| HSV-miRNA, scrambled (control) sequence | This study | |
| E. coli (HT115 (F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lacUV5 promoter -T7 polymerase)) expressing empty vector (L4440) or different RNAi-encoding plasmids (C. elegans RNAi Collection (Ahringer)) | Source BioScience | SCR_017064 |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| [1,2-13C2]-d-glucose | Sigma–Aldrich | Cat.No. 453188 |
| [U–13C6]isoleucine | Sigma–Aldrich | Cat.No. 604798 |
| [U–13C5]valine | Sigma–Aldrich | Cat.No. 758159 |
| [13C2,15N]-GSH | Sigma–Aldrich | Cat.No. 683620 |
| Tetramethylrhodamine ethyl ester (TMRE) | Fisher Scientific | Cat. No. T669 |
| Antimycin A | Sigma | Cat. No. A8674 |
| Piericidin A | Cayman | Cat. No. 15379 |
| 2-deoxy-d-glucose (2-DG) | Cayman 14325 | Cat. No. 14325 |
| carbonyl cyanide m-chlorophenyl hydrazone (CCCP) | Sigma | Cat. No. C2759 |
| Oligomycin A | Sigma | Cat. No. 75351 |
| Critical commercial assays | ||
| NAD+/NADH | BioVision | #K337 |
| NADP+/NADPH | BioVision | #K347 |
| Deposited data | ||
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Time-pregnant female rats (Sprague–Dawley) – Charles River | ||
| C. elegans strain: (eri-1(mg366) IV; uls60[P-unc-119::YFP + P-unc-119::sid-1]; dvIs62(snb-1::TDP-43)X) | Christopher LInk | CL6306 |
| C. elegans strain: eri-1(mg366) IV) | Christopher LInk | CL6186 |
| C. elegans strain: (uls60[P-unc-119::YFP + P-unc-119::sid-1]) | Christopher LInk | CL6264 |
| C. elegans strain: (eri-1(mg366) IV; uls60[P-unc-119::YFP + P-unc-119::sid-1]) | This study | RK179 |
| BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) | Horizon Discovery | YSC1048 |
| BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xxxΔ0) | Horizon Discovery | YSC1053 |
| BY4743 (MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 LYS2/lys2Δ0 ura3Δ0/ura3Δ0) | Horizon Discovery | YSC1050 |
| BY4743 (MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 LYS2/lys2Δ0 ura3Δ0/ura3Δ0 xxx/xxxΔ0) | Horizon Discovery | YSC1057 |
| Oligonucleotides | ||
| G6pd_3_top: CCTGGAGGCTTGCTGAAGGCTGTATGCTGAGGAGTTACGGGCAAAGAACTGTTTTGGCCACTGACTGACAGTTCTTTCCGTAACTCCTCAGGACACAAGGCCTGTTACTAGCACTCACATGGAACAAATGGCCT | IDT | This study |
| G6pd_3_bottom: CCGGAGGCCATTTGTTCCATGTGAGTGCTAGTAACAGGCCTTGTGTCCTGAGGAGTTACGGAAAGAACTGTCAGTCAGTGGCCAAAACAGTTCTTTGCCCGTAACTCCTCAGCATACAGCCTTCAGCAAGCCTCCAGGCCGG | IDT | This study |
| Recombinant DNA | ||
| Rat G6pd (NM_017006.2) in pcDNA3.1+/C-(K)-DYK | Genscript | This study |
| Software and algorithms | ||
| ImageJ plugin wrMTrck | J Vis Exp 52321 | http://www.phage.dk/plugins/wrmtrck.html |
| Other | ||