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. Author manuscript; available in PMC: 2022 Mar 28.
Published in final edited form as: Cell Rep. 2022 Mar 8;38(10):110491. doi: 10.1016/j.celrep.2022.110491

Figure 3. SRC-3 regulates the hepatic 12-h lipidome. Rhythmic hepatic lipid species detected by mouse liver lipidomics and the RAIN method were plotted as heatmaps for 12- and 24-h cycling lipids.

Figure 3.

(A) Heatmaps of the 12-h-oscillating lipid species for the SRC-3 WT and KO mice (n = 506).

(B) Heatmaps of the 24-h-oscillating lipid species (n = 2,510).

(C) The proportion of the 12-h lipid oscillations that had abolished (n = 408, 81%) or sustained (n = 98,; 19%) 12-h periods in the SRC-3 KO mice (top left) and the proportion of the 24-h lipid oscillations that had abolished (n = 607, 24%) or sustained (n = 1,903, 76%) 24-h periods in the SRC-3 KO mice (bottom left). The hepatic 12-h lipid species that are disrupted in the SRC-3 KO mice and their specific types and proportion are shown on the right.

(D–H) Heatmap of the top significant 12-h lipid species (p < 0.01)that were disrupted in the SRC-3 KO mouse livers. The heatmap from 4-h resolution whole-liver lipidomic profiling (left) and the indicated lipid species (right) are shown.

(E–H) Representative 12-h lipid species phosphatidylcholine (PC; E), cardiolipin (CL; F), triglyceride (TG; G), and phosphatidylethanolamines (PE; H) plotted against ZT from 4-h-resolution hepatic lipidomic profiling of the SRC-3 WT (blue lines) and KO (red lines) mice. Relative abundance (R.A.) of peak spectra is shown.