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. Author manuscript; available in PMC: 2022 Mar 28.
Published in final edited form as: Cell Rep. 2022 Mar 8;38(10):110491. doi: 10.1016/j.celrep.2022.110491

Figure 7. Maintenance and plasticity of the cistromic 12-h clock coactivation in the mouse liver.

Figure 7.

(A–D) Binding profiles and heatmaps of XBP1s (A and B) and SRC-3 (C and D) ChIP-seqs at 13,463 hepatic binding sites under ad libitum chow-feeding condition every 4 ZT h for 24 h in total.

(E) The relative signal of XBP1s and SRC-3 ChIP-seqs plotted as normalized binding profiles every 4 ZT h for 24 h in total (normalized uniquely mapped reads in a 50-bp bin). Gray bar indicates the time of peak in the XBP1- and SRC-3 binding profiles. The 12-h rhythmic eigenvalue parameters period, phase, and relative amplitude of the XBP1/SRC-3 genomic binding profiles are shown.

(F–I) Binding profiles and heatmaps of XBP1s (F and G) and SRC-3 (H &I) ChIP-seqs at 13,463 hepatic binding sites after 16-h overnight fasting regimen every 4 ZT h for 24 h in total.

(J and K) The relative signal of XBP1s (J) and SRC-3 (K) ChIP-seqs in chow versus fasting conditions plotted as normalized binding profiles every 4 ZT h for 24 h in total (normalized uniquely mapped reads in a 50-bp bin). The phases of XBP1 binding in chow condition (gray bar), XBP1 binding in fasting condition (orange bar), SRC-3 binding in chow condition (blue bar), and SRC-3 binding in fasting condition (purple bar) are shown.

(L) 12-h rhythmic eigenvalue parameters period, phase, amplitude (relative), amplitude (RPKM), mean (RPKM), and decay rate of the XBP1/SRC-3 genomic binding profiles in chow and fasting conditions are shown.