KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-SRC-3 | Cell Signaling Technology | Cat# 2126, RRID: AB_823642 |
| Rabbit polyclonal anti-XBP1s | Biolegend | Cat# 619501, RRID: AB_315907 |
| Rabbit monoclonal anti-ATF4 | Cell Signaling Technology | Cat# 11815S, RRID: AB_2616025 |
| Rabbit polyclonal anti-ATF6-α | Santa Cruz Biotechnology | Cat# sc-22799, RRID: AB_2242950 |
| Rabbit polyclonal anti-XBP1 | Santa Cruz Biotechnology | Cat# sc-7908, RRID: AB_2107620 |
| Rabbit polyclonal anti-BMAL1 | Abcam | Cat# ab3350, RRID: AB_303729 |
| Rabbit anti-beta-Actin Monoclonal | Cell Signaling Technology | Cat# 5125, RRID: AB_1903890 |
| Antibody, HRP Conjugated, Clone 13E5 | ||
| Bacterial and virus strains | ||
| Eif2ak3 promoter-pGreenfire-mCMV-dscGFP-EF1-Puro lentivirus | (Zhu et al., 2017) | N/A |
| XBP1s-pHAGE | A gift from Dr. Xi Chen at BCM | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Tunicamycin | EMD Millipore | Cat# 654380 |
| Eif2ak3 promoter-pGreenfire-mCMV-dscGFP-EF1-Puro lentivirus | (Zhu et al., 2017) | N/A |
| Hygromycin B | Thermo Fisher Scientific | Cat# 10687010 |
| Lipofectamine® 2000 Transfection Reagent | Thermo Fisher Scientific | Cat# 11668027 |
| Lipofectamine® RNAiMAX Transfection Reagent | Thermo Fisher Scientific | Cat# 13778030 |
| HEPES | Thermo Fisher Scientific | Cat# 15630080 |
| SuperScript® VILO™ Master Mix | Thermo Fisher Scientific | Cat# 11755050 |
| Micrococcal Nuclease | New England Biolabs | Cat# M0247S |
| Sodium Pyruvate | Thermo Fisher Scientific | Cat# 11360070 |
| PEG-it Virus Precipitation Solution | System Biosciences | Cat# LV810A-1 |
| RNase A, DNase and protease-free | Thermo Fisher Scientific | Cat# EN0531 |
| TaqMan® Universal Master Mix II, no UNG | Thermo Fisher Scientific | Cat# 4440047 |
| Power SYBR Green Master Mix | Thermo Fisher Scientific | Cat# 4367660 |
| Dialyzed Fetal Bovine Serum | Thermo Fisher Scientific | Cat# 26400036 |
| Fetal Bovine Serum | Thermo Fisher Scientific | Cat# 10437028 |
| D-glucose | Sigma | Cat# G8270 |
| Sodium Acetate | Sigma | Cat# S2889 |
| 2-Mercaptoethanol | Sigma | Cat# M3148 |
| DMSO | Sigma | Cat# D8418 |
| NP-40 | Sigma | Cat# N3516 |
| Sodium chloride | Sigma | Cat# S7653 |
| Tris/glycine/SDS buffer | Biorad | Cat# 161-0772 |
| TBST | Cell Signaling Technology | Cat# 9997 |
| 10% SDS | Gibco | Cat# 15553-035 |
| Triton X-100 | Promega | Cat# H5142 |
| Tween 20 | Fisher Scientific | Cat# BP337-100 |
| BSA | Sigma | Cat# A9647 |
| EDTA | Cell Signaling Technology | Cat# 7011S |
| Nonfat dry milk | Biorad | Cat# 170-6404 |
| BCA Protein Assay Reagent A | Thermo Fisher Scientific | Cat# 23223 |
| BCA Protein Assay Reagent B | Thermo Fisher Scientific | Cat# 23224 |
| iBlot Gel transfer stacks Nitrocellulose | Life Technologies | Cat# IB301001 |
| Sodium deoxycholate | Sigma | Cat# D6750 |
| Mouse Genomic DNA | Promega | Cat# G3091 |
| Ponceau S solution | Sigma | Cat# P7170 |
| VivoGlo™ Luciferin, In Vivo Grade | Promega | Cat# P1041 |
| Restore western blot stripping buffer | Thermo Fisher Scientific | Cat# 21063 |
| Penicillin Streptomycin | Thermo Fisher Scientific | Cat# 15070063 |
| Pellet Paint® Co-Precipitant | EMD Millipore | Cat# 69049 |
| Q5® High-Fidelity DNA Polymerase | New England Biolabs | Cat# M0491S |
| Criterion pre-cast gradient SDS-PAGE gel (4-20%) | Bio-Rad | Cat# 5671094 |
| TaKaRa Ex Taq® DNA Polymerase | Clontech | Cat# RR001A |
| T4 DNA Ligase | New England Biolabs | Cat# M0202 |
| Antarctic Phosphatase | New England Biolabs | Cat# M0289S |
| Ethanol 200 proof | Decon Labs | Cat# 2701 |
| Isopropanol | Sigma | Cat# I9516 |
| cOmplete™ Protease Inhibitor Cocktail | Roche | Cat# 11697498001 |
| PhosSTOP™ | Roche | Cat# 4906845001 |
| DMEM, high glucose | Thermo Fisher Scientific | Cat# 11965092 |
| DMEM, no glucose | Thermo Fisher Scientific | Cat# 11966025 |
| Proteinase K | Thermo Fisher Scientific | Cat# AM2544 |
| 0.45μM syringe filter | Thermo Fisher Scientific | Cat# 09-740-106 |
| Critical commercial assays | ||
| SimpleChIP® Plus Enzymatic Chromatin IP Kit | Cell Signaling Technology | Cat# 9005S |
| PureLink RNA mini kit | Life Technologies | Cat# 12183025 |
| Dual-luciferase Reporter assay system | Promega | Cat# E1960 |
| Insulin ELISA kit | EMD Millipore | Cat# EZRMI-13K |
| One touch ultra glucose meter and strips | LifeScan, Inc | N/A |
| Deposited data | ||
| GSE175598 | GEO public repository | GSE175598 |
| Study ST002079/PR001319 | NMDR public repository | datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench.org:22222/data/DRCCMetadata.php?Mode=Study&StudyID=ST002079&Access=DoqF5000 |
| PASS01737 | PeptideAtlas (ProteomeXchange consortium member) | PASS01737; http://www.peptideatlas.org/PASS/PASS01737 |
| Experimental Models: Cell Lines | ||
| Immortalized mouse embryonic fibroblasts | (Zhu et al., 2017) | N/A |
| Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs | (Zhu et al., 2017) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J male mice | The Jackson Laboratory | JAX:000664 |
| Mouse: SRC-3 knockout male mice | (Xu et al., 2000) | N/A |
| Mouse: Xbp1 flx/flx male mice | A gift from Dr. Xi Chen at BCM (Lee et al., 2008) | N/A |
| Mouse: Xbp1 flx/flx;AlbCre male mice | (Meng et al., 2020) | N/A |
| Oligonucleotides | ||
| siRNA oligonucleotides | Dharmacon | Dharmacon, D-001206-13-05; Dharmacon, L-040825-00-0005 |
| See Table S5 for qRT-PCR and ChIP-qPCR Primer Sequences | N/A | N/A |
| Recombinant DNA | ||
| Eif2ak3-dluc | (Zhu et al., 2017) | N/A |
| Eif2ak3-dluc mutant | (Zhu et al., 2017) | N/A |
| Software and algorithms | ||
| RAIN | (Thaben and Westermark, 2014) | http://www.bioconductor.org/packages/release/bioc/html/rain.html |
| Eigenvalue/pencil | (Antoulas et al., 2018; Zhu et al., 2017) | N/A |
| Bioconductor 3.4 | R | https://www.bioconductor.org/news/bioc_3_4_release/ |
| Matlab R2019a | MathWorks | https://www.mathworks.com/products/new_products/latest_features.html |
| GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| LumiCycle Analysis 2.54 | ActiMetrics | http://actimetrics.com/downloads/lumicycle/ |
| UCSC genome browser | UCSC | https://genome.ucsc.edu/cgi-bin/hgGateway |
| DAVID Bioinformatics Resources 6.8 | NIH | https://david.ncifcrf.gov/ |
| CLAMS data examination tool | Columbus Instruments | http://www.colinst.com/products/clams-hc-comprehensive-lab-animal-monitoring-system-for-home-cages#CLAX |
| VirtualDubMod 1.5.10 | Intel Software | https://sourceforge.net/projects/virtualdubmod/files/ |
| Venn Diagram Plotter v1.5.5228.29250 | Department of Energy and PNNL | https://omics.pnl.gov/software/venn-diagram-plotter |
| Gene Cluster 3.0 | Originally writtern by Michael Eisen at Stanford University | http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm#ctv |
| Java TreeView 3.0 alpha03 | Open Source software | https://bitbucket.org/TreeView3Dev/treeview3/ |
| Other | ||
| NCBI Aceview (AceView offers a comprehensive annotation of human, mouse and nematode genes) | NIH | https://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/index.html |