Fig. 2.

Phylogenetic tree of Borrelia taxa based on flagellin gene (158 nt). DNA extracts from two different areas of the liver biopsy were analyzed (indicated in red). The phylogenetic analyses were generated with the BioNeighbor-Joining method. The phylogeny presented is based on the alignment of 158-bp sequences of flaB gene from 44 reference sequences representing the current known diversity in the genus Borrelia and the sequence amplified in the case report presented herein. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. The tree is not rooted and drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter method and are represented in the units of the number of base substitutions per site. Evolutionary analyses were conducted using SeaView. All sequences are labeled by species, strain name, and GenBank accession number. TS type strain