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. 2021 Aug 4;19(3):343–357. doi: 10.20892/j.issn.2095-3941.2020.0661

Figure 3.

Figure 3

The m6A writers maintained the expression of stemness-related genes in inflammatory responses. (A) Venn diagram for the aberrant genes in the sh-METTL3, sh-METTL14, and sh-WTAP samples. (B) Heat map for the top 30 differentially-expressed stemness-related genes in m6A gene knocked-down cells. (C) The qRT-PCR showing the expression levels of METTL3, METTL14, CD133, CTNNB1, and SOX6 in METTL3 or METTL14-silenced CCAs. Error bars denote ± SEM (*P < 0.05; ***P < 0.001) in 3 independent experiments. (D) Knockdown of METTL3 or METTL14 reduced the CTNNB1 and SOX6 protein levels. The METTL3/ACTIN, METTL14/ACTIN, CTNNB1/ACTIN, or SOX6/ACTIN densitometric ratio was recorded using ImageJ software. (E) The qRT-PCR showing the expression levels of CD133 and CTNNB1 in the m6A writer knockdown cells (with or without 20 ng/mL IL-6 exposure). Error bars denote ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001) in 3 independent experiments.