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. 2021 Aug 4;19(3):343–357. doi: 10.20892/j.issn.2095-3941.2020.0661

Figure 4.

Figure 4

The m6A writers regulate the expression of stemness-related genes in a m6A depended manner. (A, B) METTL3 (A) and METTL14 (B) knockdown reduced the enrichment of m6A modifications of CTNNB1, SOX4, and SXO6. Error bars denote ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001) in 3 independent experiments. (C, D) IGF2BP2 (5.9-fold changes; P < 0.0001), YTHDC1 (1.7-fold changes; P = 0.0423), ELAVL1 (1.6-fold changes; P = 0.0096), and YTHDC2 (P = 0.4379) (C), and CTNNB1 (P = 0.0002) (D) are highly expressed in cancerous tissues compared to those in matched adjacent tissues of 38 paired CCA patient samples. Wilcoxon matched-pairs signed rank test was used to calculate the significance. BB indicates CCA adjacent tissue, and BC indicates CCA cancerous tissue. (E) The enrichment of IGF2BP2 on CTNNB1 mRNA in the METTL3 knockdown cells treated with or without IL-6. Error bars denote ± SEM (***P < 0.001) in 3 independent experiments, using HPRT1 as the negative control.