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(A) Cytoplasmic recycling pathway for MurNAc in P. putida. Since this pathway is not present in many bacteria, it must be introduced via genetic engineering in those cases. (B) Representative MurNAc derivatives for labeling the PG glycan core, modified at the N-acyl group (B) and the D-Lac group (C). (D) Chemical synthesis of N-acyl-modified MurNAc derivatives 41–46 and chemoenzymatic synthesis of their corresponding sugar phosphate and UDP sugar derivatives (box).
