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. Author manuscript; available in PMC: 2023 Feb 9.
Published in final edited form as: Chem Rev. 2021 Dec 14;122(3):3336–3413. doi: 10.1021/acs.chemrev.1c00729

Figure 27.

Figure 27.

(A) Chemoenzymatic synthesis of AltNAc4NAz (75), a chemical reporter for pseudaminic acid-containing flagellin glycoprotein in C. jejuni. (B) Analysis of AltNAc4NAz-labeled flagellin from C. jejuni by Western blot. Wild-type or PseG-deficient C. jejuni were grown on agar containing 75, reacted with phosphine-biotin, lysed, and probed by streptavidin-horseradish peroxidase (HRP) or anti-flagellin Western blot. For streptavidin-HRP blot: lane 1, C. jejuni ΔpseG + 75; lane 2, C. jejuni ΔpseG no probe; lane 3, C. jejuni wild type no probe; lane 4, C. jejuni wild type + 75. For anti-flagellin blot: lane 1, C. jejuni ΔpseG + 75; lane 2, C. jejuni ΔpseG no probe; lane 3, C. jejuni wild type no probe. Reprinted with permission from ref 202. Copyright 2009 Wiley-VCH.