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. Author manuscript; available in PMC: 2023 Feb 9.
Published in final edited form as: Chem Rev. 2021 Dec 14;122(3):3336–3413. doi: 10.1021/acs.chemrev.1c00729

Figure 40.

Figure 40.

Imaging of mycoloyltransferase hydrolytic activity in mycobacteria using FRET-based fluorogenic QTF. (A) Upon hydrolysis by mycoloyltransferases (e.g., Ag85), the ester bond of QTF (125) is cleaved, releasing products 126 and 127, the former of which is fluorescent. (B) A mixed culture of mCherry-expressing C. glutamicum (red), B. subtilis, and E. coli was incubated in the presence of 1 μM QTF, fixed, and imaged by confocal fluorescence microscopy. An overlay of all channels is shown. Scale bar, 5 μm. Reproduced from ref 276.