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. Author manuscript; available in PMC: 2023 Feb 9.
Published in final edited form as: Chem Rev. 2021 Dec 14;122(3):3336–3413. doi: 10.1021/acs.chemrev.1c00729

Figure 42.

Figure 42.

Analysis of Tdmh activity in mycobacteria using fluorogenic FRET-TDM. (A) Upon hydrolysis by Tdmh, an ester bond of FRET-TDM (128) is cleaved. Either ester bond of FRET-TDM can be cleaved by Tdmh; the possibility in which products 119 and 129 are generated is shown. (B) Recombinant purified wild-type Tdmh or its active site mutant S124A were resolved by native PAGE on two separate gels, one of which was Coomassie-stained (left) and the other was treated with 10 μM FRET-TDM (128) and scanned for 488 fluorescence (right). Reproduced with permission from ref. 277 (https://pubs.acs.org/doi/10.1021/acsomega.9b00130). Copyright 2019 American Chemical Society. Further permission related to the material excerpted should be directed to the American Chemical Society.