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. 2022 May 3;8(2):00652-2021. doi: 10.1183/23120541.00652-2021

FIGURE 2.

FIGURE 2

Loss of glycocalyx in primary human pulmonary microvascular endothelial cells in the presence of serum of coronavirus disease 2019 (COVID-19) patients in the intensive care unit (ICU). a) Representative fluorescence (FL) confocal images of Lycopersicon esculentum (LEA)-fluorescein isothiocyanate (FITC) or anti-thrombomodulin (TM) staining on the surface of primary human pulmonary microvascular endothelial cells (HPMECs) in the presence of 10% serum of pooled healthy controls (n=12), COVID-19 non-ICU (n=8) and COVID-19 ICU (n=26) samples for 24 h. HPMEC surface expression quantification of b) LEA-FITC and c) TM in the presence of 10% pooled healthy control, COVID-19 non-ICU and COVID-19 ICU serum for 24 h. All values are given as mean±sd of four independent experiments. One-way ANOVA followed by Tukey's multiple comparisons test was performed. ***: p<0.001.