Table 3. Reactivation Kinetics of hAChE Inhibited by Nerve Agent Surrogated and Paraoxon by Selected Oximes^a.

OP	oxime	kr min–1	KD mM	kr2 mM–1·min–1
NEMP	1	0.4 ± 0.1	4 ± 1	0.1
	2	0.3 ± 0.01	1 ± 0.09	0.2
	3	0.5 ± 0.07	1.5 ± 0.3	0.3
	4	1 ± 0.1	1 ± 0.3	1
	4’	1 ± 0.02	1 ± 0.04	1
	5	2.5 ± 0.2	2.4 ± 0.4	1.0
	HI-6	0.65 ± 0.03	0.07 ± 0.008	9.3
	2-PAM	0.1 ± 0.01	0.2 ± 0.06	0.5
	obidoxime	0.2 ± 0.007	0.6 ± 0.04	0.3
NIMP	1	0.2 ± 0.006	1 ± 0.08	0.2
	2	0.2 ± 0.02	2 ± 0.4	0.1
	3	0.2 ± 0.01	1.3 ± 0.2	0.15
	4	0.7 ± 0.1	2 ± 0.5	0.4
	4’	0.4 ± 0.09	2 ± 0.7	0.2
	5	nd	nd	0.2
	HI-6	1 ± 0.2	0.09 ± 0.03	11
	2-PAM	0.24 ± 0.02	0.2 ± 0.03	1.2
	obidoxime	0.2 ± 0.02	0.5 ± 0.09	0.4
NEDPA	1	0.1 ± 0.01	1.3 ± 0.2	0.1
	2	0.2 ± 0.02	1.2 ± 0.3	0.2
	3	0.2 ± 0.08	3 ± 1.4	0.07
	4	0.6 ± 0.02	0.9 ± 0.1	0.6
	4’	0.5 ± 0.04	0.6 ± 0.1	0.8
	5	0.8 ± 0.2	3 ± 1	0.3
	HI-6	0.05 ± 0.005	0.2 ± 0.04	0.2
	2-PAM	0.15 ± 0.025	0.8 ± 0.2	0.2
	obidoxime	0.6 ± 0.04	0.3 ± 0.07	2
PARAOXON	1	0.03 ± 0.005	0.5 ± 0.2	0.05
	2	nd	nd	0.2
	3	0.2 ± 0.08	2 ± 0.4	0.2
	4	0.6 ± 0.1	2 ± 0.7	0.3
	4’	nd	nd	0.8
	5	0.6 ± 0.1	2.7 ± 0.7	0.2
	HI-6	0.09 ± 0.009	0.8 ± 0.14	0.1
	2-PAM	0.05 ± 0.006	0.4 ± 0.08	0.1
	obidoxime	0.3 ± 0.02	0.5 ± 0.09	0.6

^and: If [reactivator] ≪ K_D, then there is a linear dependence between k_obs and [reactivator]: k_obs = (k_r/K_D)[reactivator]. In this case, k_r and K_D cannot be determined, but k_r2 = k_r/K_D is the slope of the line and can be directly obtained by fitting.