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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2360:253–277. doi: 10.1007/978-1-0716-1633-8_19
Component uL Concentration
Q5 high-fidelity 2X 25 1 X
Forward Primer T7 X 2.5 0.5 μM
Reverse Primer T7 X 2.5 0.5 μM
Template DNA (gBlock) 2 <1.000 ng
DNase-RNase free-water 18
TOTAL VOL 50