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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2360:253–277. doi: 10.1007/978-1-0716-1633-8_19
Component μL
PCR + gBlock (2 μg) X
10x T7 Reaction Buffer 2
ATP Solution 2
CTP Solution 2
GTP Solution 2
UTP Solution 2
T7 Enzyme Mix 2
Nuclease-Free Water X
TOTAL VOL 20 μL