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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2360:253–277. doi: 10.1007/978-1-0716-1633-8_19
Component μL
dsRNA reaction from Step 4 50
10X Binding buffer 50
Nuclease-free water 150
100% Ethanol 250
Total Volume 500 μL
Gently mix the reaction by pipetting up and down.