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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2360:253–277. doi: 10.1007/978-1-0716-1633-8_19
Compound μL
T7 10x Reaction buffer 2
T7 ATP solution 2
T7 CTP solution 2
T7 GTP solution 2
T7 UTP solution 2
DNA template (PCR: 2μg) X
Nuclease free water. X
T7 Enzyme Mix 2
TOTAL VOL 20