Table 1.
List of genes targeted by RNAi and dsRNA delivery methods in different insect orders
| dsRNA Delivery Methods | ||||||
|---|---|---|---|---|---|---|
| Orden | Specie | Target | Oral | Injection/Soaking | Stage | Ref |
| Coleoptera | Tribolium castaneum | V-ATPase E | Artificial diet | Neonates | (49) | |
| Chewing-feed | Diabrotica virgifera | Multiple targets | Artificial diet | Neonates | (68) | |
| Virgifera | Snf 7 | Artificial diet | Neonates | (15) | ||
| Bol and vgr | Transgenic Plant | Larva/Adults | (69) | |||
| Leptinotarsa | Multiple targets | Leaf tissue | Neonates | (68) | ||
| decemlineata | V-ATPase A and E | TransgenicEcoli | 2nd Instar | (70) | ||
| Diabrotica | Snf 7 | Artificial diet | Neonates | (15) | ||
| undecimpuctata | V- ATPase A and E | Artificial diet | Neonates | (68) | ||
| howardi | α-Tubulin | Artificial diet | Neonates | (68) | ||
| Phyllotreta striolata | Arginine kinase | Leaf tissue | Adults | (71) | ||
| Diptera | Aedes aegypti | V-ATPase A Multiple targets | Artificial diet | Adults | (72) | |
| Water | 1st Instar | (50) | ||||
| Blood-nectar plant-insect juice Sucking-Feed |
ATP-dependent efflux | Water | 2nd Instar | (73) | ||
| Pump | ||||||
| Anhopeles gambiae | Chitin synthase 1, 2 | Artificial diet | 3rd Instar | (74) | ||
| Anopheles stephensi | 3-HKT | Transgenic algae | 1st Instar | (75) | ||
| Bactrocera dorsalis | Multiple targets | Transgenic E.coli | Adults | (76) | ||
| Glossina morsitans | Tsetse EP | Blood meal | Male Adults | (77) | ||
| Morsitans | Transferrin | Blood meal | Male Adults | (77) | ||
| Hemiptera | Acyrthosiphon pisum | Aquaporin | Artificial diet | 6-day-old nymphs | (78) | |
| V-ATPase E | Artificial diet | 1st Instar | (49) | |||
| Phloem/Xylem Sucking-feed | V-ATPase E | Artificial diet | Neonates | (79) | ||
| Hunchback (hb) | Artificial diet | Neonates | (80) | |||
| Salivary protein COO2 | Microinjection | Adults | (81) | |||
| Gut digestive enzyme cathepsin-L | Artificial diet | Microinjection | 1st, 3rd Instar | (82) | ||
| Structural sheath protein SHP | Microinjection | Adults | (83) | |||
| Angiotensin-converting enzymes ACE1 and ACE2 | Microinjection | Adults | (84) | |||
| Peroxiredoxin 1 gene ApPrxl | Microinjection | Adults | (85) | |||
| Macrophage migration inhibitory factor ApMIF1 | Microinjection | Adults | (86) | |||
| Calreticulin, Cathepsin-L | Microinjection | Adults | (87) | |||
| Myzus persicae | Rack1 and COO2 | Transgenic Plants | Adults | (88) | ||
| Aphis gossypii | Carboxylesterase geneCar | Artificial diet | Adults | (89) | ||
| Cytochrome P450 monooxygenase gene CYP6A2 | Artificial diet | 3rd instar, Adults | (90) | |||
| Odorant-binding protein 2 AgOBP2 | Artificial diet | Adults | (91) | |||
| Bactericera cockerelli | Multiple targets | Artificial diet | Adults | (92) | ||
| Bemisia tabaci | V-ATPase subunit A, rpL19 V-ATPase subunit A AChE and EcR |
Artificial diet | Adults | (93) | ||
| Transgenic Plants | Adults | (94) | ||||
| Transgenic Plants | Adults | (95) | ||||
| Nilaparvata lugens | Trehalose PO4 synthase | Artificial diet | 3rd instar | (74) | ||
| V-ATPase E | Artificial diet | 2nd Instar | (96) | |||
| Grain aphid | Catalase gene CAT | Artificial diet | 3rd instar | (97) | ||
| Acetylcholinesterase gene SaAce1 | Microinjection | Adults | (98) | |||
| Multiple targets | Artificial diet | 3rd instar | (99) | |||
| Multiple targets | Artificial diet | Adults | (100) | |||
| Olfactory coreceptor gene SaveOrco | Artificial diet | Adults | (90, 101) | |||
| Rhopalosiphum padi | Acetylcholinesterase gene RpAcel | Microinjection | Adult s | (98) | ||
| Schizaphis graminum | Salivary protein C002 | Artificial diet | Adults | (102) | ||
| Peregrinus maidis | V-ATPase B and D | Artificial diet | 3rd instar | (103) | ||
| Rhodnius prolixus | Nitrophorin 2 | Artificial diet | 2nd Instar | (104) | ||
| Lygus lineolaris | Inhibitor of apoptosis | Artificial diet | Neonates | (105) | ||
| Diaphorina citri | awd gene muscle protein 20 carboxyesterases CYP4 genes sucrose hydrolase boule homologue gene glutathione S-transferase transformer 2 homologue | Topical | 5th instar | (106) | ||
| Water | 4rd, 5th instar | (63) | ||||
| Topical | 4rd, 5th instar | (62) | ||||
| Topical | Adults | (107) | ||||
| Topical | 4–5th instar, Adults | (44) | ||||
| Artificial diet | Adults | (64) | ||||
| Artificial diet | Adults | (108) | ||||
| Artificial diet | Female Adults | (66) | ||||
| Hymenoptera | Apis mellifera | Vitellogenin | Natural diet | 2nd Instar | (109) | |
| Chewing and Sucking-Feed | Solenopsis invicta | PBAN/pyrokinin | Artificial diet | 4rd instar | (110) | |
| Guanine nucleotide binding GNBP | Artificial diet | Workers | (111) | |||
| Isoptera | Reticulitermes flavipes | Cellulase | Paper disc | Workers | (112) | |
| Chewing-Feed | Hexamerin | Paper disc | Workers | (112) | ||
| Lepidoptera | Chilo infuscatellus | CiHR3 molting factor | Corn kernels | 3rd instar | (113) | |
| Chewing and Sucking-Feed | Epiphyas postvittana | Carboxylesterase | Droplet | 3rd instar | (36) | |
| Pheromone bp | Droplet | 3rd instar | (36) | |||
| Helicoverpa armigera | AchE receptor | Artificial diet | Neonates | (114) | ||
| AchE receptor | Leaf tissue | Neonates | (114) | |||
| Ecdysone receptor EcR | Artificial diet | 3rd instar | (115) | |||
| HaHR3 molting factor | Artificial diet | 3rd instar | (116) | |||
| CYP6B6 | Artificial diet | 3rd instar | (26) | |||
| CYP6AE14 | Transgenic Plant | Adults | (117) | |||
| Ultraspiracle protein, EcR | Artificial diet | 3rd instar | (118) | |||
| Manduca sexta | V-ATPase E | Artificial diet | Neonates | (49) | ||
| Ostrinia nubilalis | Chitinase | Artificial diet | Neonates | (119) | ||
| Plutella xylostella | CYP6BG1 | Artificial diet | 4rd instar | (120) | ||
| Rieske protein | Artificial diet | 2nd Instar | (121) | |||
| AchE receptor | Artificial diet | 2nd Instar | (122) | |||
| Sesamia nonagrioides | JH esterase JHER | Droplet | 1st – 6th instars | (123) | ||
| Spodoptera exigua | Chitin synthase A β1 integrin subunit | Leaf tissue | Neonates | (124) | ||
| Leaf tissue | 4rd instar | (125) | ||||
| Spodoptera litura | Aminopeptidase N | Artificial diet (Ec) | Neonates | (126) | ||
| Bombix mori | atg gene bursicon gene | Injection | Larva | (127) | ||
| Injection | Pupae | (128) | ||||
| Spodoptera frugiperda | Allatostatin C | Droplet | Injection | 1st instar, larva | (129) | |
| Allototropin 2 | Droplet | 1st instar | (129) | |||
| SfT6 serine protease | Droplet | 4rd instar | (130) | |||
| Orthoptera | Gryllus bimaculatus | Sulfakinins | Droplet | Adults | (131) | |
| Chewing-Feed | Locusta migratoria | Multiple targets | Artificial diet | 4rd instar | (132) | |
| Schistocerca gregaria | Tubulin, GAPDH | Artificial diet | Adults | (133) | ||
Modified 744 of Kumar et al., 2018