Table 1 |.
Competitive binding of TTX by peptides as determined by TTX eLiSA
| Molar ratios of componentsa | Groups | TTX detected (ng ml–1) | TTX detected (%) | TTX bound by peptide (%) |
|---|---|---|---|---|
| 0:0:1 | No peptide | 270.0 ± 3.2 | 100.0 ± 1.2 | 0.0 ± 1.2 |
| 1:1:1 | P1P2 | 252.9 ± 8.2 | 93.7 ± 3.0 | 6.3 ± 3.0 |
| ϕFFF-P1P2 | 92.8 ± 13.2 | 34.4 ± 4.9 | 65.6 ± 4.9 | |
| ϕYFY-P1P2 | 236.2 ± 9.5 | 87.5 ± 3.5 | 12.5 ± 3.5 | |
| 5:5:1 | P1P2 | 242.7 ± 4.8 | 89.9 ± 1.8 | 10.1 ± 1.8 |
| ϕFFF-P1P2 | 15.4 ± 6.8 | 5.7 ± 2.5 | 94.3 ± 2.5 | |
| ϕYFY-P1P2 | 21.2 ± 9.1 | 7.8 ± 3.4 | 92.2 ± 3.4 | |
| ϕFFF-MuDP1P2 | 69.9 ± 11.7 | 25.9 ± 4.3 | 74.1 ± 4.3 | |
| ϕFFF-MuQP1P2 | 233.6 ± 10.3 | 86.5 ± 3.8 | 13.5 ± 3.8 | |
| 10:1 | ϕFFF-P1P1 | 72.1 ± 12.2 | 26.7 ± 4.5 | 73.3 ± 4.5 |
| ϕFFF-P2P2 | 102.2 ± 9.8 | 37.8 ± 3.6 | 62.2 ± 3.6 |
In all groups, the concentration of TTX added was 270 ng ml−1 (0.9 μM). n = 4 independent experiments, data are shown as the mean ± s.d.
a
Ratio of P1-containing peptide:P2-containing peptide:TTX (or peptide:TTX).