Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 8;13:866641. doi: 10.3389/fendo.2022.866641

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Liang, Zhao, Dou, Yang, Zhao, Zhou, Zhang, Yang and Zeng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Isolation and culture of rat primary BMSCs, the induction of osteogenic and adipogenic differentiation and the detection of cell surface markers. (A) The morphology of primary BMSCs from third-generation rats was observed under a microscope (×40). (B) After the osteogenic differentiation of third-generation BMSCs was induced with an osteogenic differentiation-inducing agent (Alizarin red staining), the formation of mineralized nodules was observed under a microscope (×100). (C) The adipogenic differentiation of third-generation BMSCs was induced by an adipogenic differentiation-inducing agent, and lipid droplets were enlarged and made rounder by continuous culture with maintenance medium (stained with oil red O) and observed under a microscope (×200). (D–F) The primary BMSCs of third-generation rats were collected and stained for CD29 (D), CD44 (E), CD45 (F) and CD90 (G) for 30 min. Control staining was simultaneously carried out. Fluorescence was detected with a flow cytometer, and the results were analysed.