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. 2022 Feb 28;149(5):dev200079. doi: 10.1242/dev.200079

Fig. 2.

Fig. 2.

Temperature-enhanced graft formation requires PIF4 and YUCs in the cotyledon. (A) Proportion of grafted pif4-2 or wild-type Arabidopsis that transported CFDA to the rootstock 3-4 DAG and recovered at 20°C or 27°C [±standard error of a proportion (s.e.p.); n=30 plants per temperature per time point]. (B) Proportion of three genotype pif4-2 grafts with fluorescent rootstocks (±s.e.p.; n=indicated on plot). (C) Relative expression levels of YUC8 and PIF4 in Col-0 or pif4-2 cotyledons after 48 h treatment of 20°C or 27°C (mean±s.d. of three biological replicates). (D) GUS histochemical staining of 8-day-old pYUC8::GUS seedlings incubated at 20°C or 27°C for 48 h. Scale bars: 1 mm. (E) Proportion of grafted yuc2/5/8/9 or wild-type Arabidopsis that transported CFDA to the rootstock, recovered at 20°C or 27°C and measured 3-4 DAG (±s.e.p.; n=30 plants per temperature per time point). *P<0.05, **P<0.01, ***P<0.001; Fisher's exact test (A,B,E) or unpaired two-tailed Student's t-test (C) compared with 20°C.