Table 1.
Methods for CTCs isolation divided by the enrichement strategy.
| Enrichment Strategy | Technology | Selection Criteria | Ref. |
|---|---|---|---|
| Immunomagnetic enrichment | |||
| Immunomagnetic positive enrichment | CellSearch® | EpCam | (66) |
| MagSweeper | EpCam | (95) | |
| Magnetic Activated Cell Sorter (MACS) | EpCam | (96) | |
| Strep-Tag | EpCam, EGFR, HER2 | (97) | |
| Immuno-magnetosomes (IMS) | EpCam | (98) | |
| AdnaTest | EpCam | (99) | |
| Immunomagnetic negative enrichment | EasySep | CD45 | (100) |
| RosetteSep | CD45 | (101) | |
| Microfluidic positive capture | CTC-Chip | EpCam | (102) |
| Isoflux | EpCam | (103) | |
| Nanovelcro | EpCam | (104) | |
| High-throughput Microsampling Unit (HTMSU) | EpCam | (105) | |
| Verifast | EpCam | (106) | |
| Size-based enrichment | |||
| Microfluidics | Parasortix | Gap sizes from 10 μm down to 4.5 μm | (107) |
| Microcavity Array (MCA) | 8-μm circular cavities | (108) | |
| Density-based | OncoQuick | Density | (109) |
| AccuCyte | Density | (110) | |
| Membrane filtration | Isolation by Size of Tumor cells (ISET) | 8 μm pores | (111) |
| Flexible micro spring array (FMSA) | 8 μm pores | (112) | |
| ScreenCell | 6.5 µm pores | (113) | |
| Fluid Assisted System Technology (FAST) | 8 μm pores | (114) | |
| Dielectrophoresis | |||
| Dielectric properties | ApoStream | Polarizability | (115) |
| In vivo | |||
| Therapeutic apheresis | Diagnostic leukapheresis | EpCam | (116) |
| GILUPI CellCollector | EpCam | (117) | |