TABLE 2. Comparison of the sensitivity of the genus-specific assays and the species-specific assays for detecting Plasmodium, by species.
|
Test and sensitivity |
Comparison of genus-specific assays |
|||||||
|---|---|---|---|---|---|---|---|---|
|
Quantitative PCR | ||||||||
|
P. falciparum |
P. vivax |
P. malariae |
P. ovale |
|||||
|
Positive |
Negative |
Positive |
Negative |
Positive |
Negative |
Positive |
Negative |
|
|
alt-Gen |
18 |
6 |
29 |
0 |
6 |
0 |
ND |
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
|
|
|
Sensitivity (95% CI) |
100% (82.41% to 100%) |
100% (88.3% to 100%) |
100% (60.97% to 100%)b |
ND |
||||
|
ƙ |
0.5 |
1.0 |
1.0 |
|
||||
|
McNemar’s test |
P = 0.04a |
NA |
NA |
|
||||
|
Test and sensitivity |
Comparison of species-specific assays |
|||||||
|---|---|---|---|---|---|---|---|---|
|
Nested PCR | ||||||||
|
P. falciparum |
P. vivax |
P. malariae |
P. ovale |
|||||
|
Positive |
Negative |
Positive |
Negative |
Positive |
Negative |
Positive |
Negative |
|
|
alt-S&T |
31 |
0 |
31 |
0 |
30 |
0 |
6 |
0 |
|
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Sensitivity (95% CI) |
93.94% (80.39% to 98.32%) |
100% (88.97% to 100%) |
100% (86.65% to 100%) |
100% (60.97% to 100)b |
||||
|
ƙ |
0.8 |
1.0 |
1.0 |
1.0 |
||||
|
McNemar’s test |
P = 0.4795 |
NA |
P = 1.00 |
NA |
||||
alt-Gen: RealStar® Malaria PCR Kit 1.0; alt-S&T: RealStar® Malaria Screen & Type PCR Kit; CI: confidence interval; NA: not applicable; ND: not done; PCR: polymerase chain reaction.
The result is statistically significant.
The large width of the confidence interval is due to the small number of samples.
Source: Table 2 was created by the authors based on the results of the study.