FIGURE 5.

2-HOBA attenuates MI-induced cardiomyocytes apoptosis. (A–F), C57BL/6 treated with vehicle, 2-HOBA or 4-HOBA, respectively, were subjected to left anterior descending artery (LAD) ligation or the sham procedure as described in Methods. (A), In situ TUNEL staining of border areas of MI-operated hearts as well as from sham controls. Scale bars: 100 μm. (B), Quantification of apoptosis cells (n = 5). (C), expression of apoptosis-related proteins including BCL-2 and Bax in myocardial tissue lysates harvested from peri-infarct areas of post-MI hearts. (F), Quantified data of immunoblotting band intensity (n = 5). **p < 0.01, ****p < 0.0001; ns, non-significant. All values are mean ± SEM.